Fusion Protein Modeling Service

Fusion protein refers to a new type of protein produced by fusing the Fc segment of immunoglobulin (lgG, lgA, etc.) with a certain biologically active functional protein molecule using genetic engineering technology. Functional proteins with biological activity can be cellular FDF factors, toxins, receptors, enzymes, antigen peptides and the like. The fusion protein can not only exert the biological activity of the fusion protein, but also has some antibody properties, such as antibody-dependent cell-mediated cytotoxicity (ADCC), complement-dependent cytotoxicity (CDC) and antibody-dependent cell-mediated cytotoxicity. Guided phagocytosis (ADCP) and so on. Protein drugs have a short half-life in plasma. In order to achieve therapeutic effects, large doses are required, which will cause serious side effects and cause a huge burden on patients. The fusion of functional protein with the Fc segment of immunoglobulin can prolong the half-life of the drug in plasma and reduce the immunogenicity of the drug, which is of great significance in the diagnosis and treatment of diseases.

Figure 1. Fusion protein.

Functions of fusion protein

• The fusion protein consists of the Fc segment of immunoglobulin and a protein molecule with biological activity. The Fc segment and the functional protein have relatively independent structural domains and functions, which can affect the physical and chemical properties and biological activities of the fusion protein from different angles.
• The Fc segment can increase molecular volume, reduce renal clearance, and extend the half-life to a certain extent.
• The fusion protein can be linked to form a stable dimer through the disulfide bond of the Fc chain region. Through further genetic engineering of disulfide bonds, the Fc fusion protein can also form a more stable hexamer complex. In addition, the Fc region can be folded independently to ensure the stability of the molecule.
• The fusion of the Fc segment with the functional protein can increase the expression and secretion of the protein in mammals. At the same time, the Fc segment can specifically bind to protein A, which is conducive to the purification of the fusion protein.

Overall solutions

CD ComputaBio's structural modeling program requires the selection of two protein components to be fused, the structure of which is known, or the use of appropriately selected templates for computational modeling.Then, the connection between the two fusion partners and the federation or domain insertion strategy are used to create the initial fusion model. The final model is generated by calculating the conformation of the region bridging the fusion partner, so that the protein domains are moved, rotated and interacted to achieve a stable conformation. The construction of the fusion protein is divided into the following five steps:

• Step 1: Determine the vector.
• Step 2: Ensure that the necessary conditions for the plasmid expression vector are met, including the selection marker, origin of replication, promoter, initiation codon, multiple cloning sites and transcription terminator.
• Step 3: T7 promoter system is a commonly used expression system, including T7 promoter, ribosome binding site, initiation codon, multiple cloning sites and transcription terminator.
• Step 4: T7 promoter RNA polymerase is expressed in the genome of the special strain. The polymerase gene is integrated into the bacterial genome in the phage lysogen pathway and induced by IPTG.
• Step 5: After constructing the fusion protein gene on the expression vector, transfer it to the host bacteria, and express the fusion protein under the induction of IPTG.

Services items

CD ComputaBio's fusion protein modeling service can reduce the cost of subsequent experiments. Fusion protein modeling service is a personalized and customized innovative scientific research service. Before determining the corresponding analysis plan and price, each project needs to be evaluated. If you want to know more about service prices or technical details, please feel free to contact us. If you want to know more about service prices or technical details, please feel free to contact us.